लण्डन-काठमाडौं

Tireed

Tireed is what i feel right now
"Its so graceful:"
"Its so beautiful:"
"Its so amazing:"
People would say
At this sight.
This sight that i am beholding.
This beautiful sight.
They are right on their terms.
It is beautiful.
This sunset.
This golden radiance it spread.
Everything became beautiful.
The leaves were gold.
The path was gold.
The clouds looked like fire.
The birds were happy
Birds:
Returning home
The animals were happy
From the day's food
And for the night's rest
All were enjoying
Only i couldnt.
Walking down the road alone
I hear laughs
I hear talks
Of them people
Happy people
Enjoying the sunset
Only i couldn't bring myself to be happy
"Loook mommy preetty"
A little boy said pointing at the setting sun
"Ohhh those eyes"
Those innocent ignorant eyes of that kid
All they knew was happiness
Its true that the sunset was beautiful
Only i couldnt think it that way
And so clouldnt you
Right:
My fren "sunset":
Though everbody is haappy
U and i know it.
Only u and i kno
Whats its like to know when ones brilliance is at end
Whats it like to be added set at the back
"Hummphh"
"Sunset"
There is nothing beautiful about it
Nothing at all
It just reminded that night is near by
Darkness is nearby
Its coming
Isnt it my fren.
You who can
Comprehend my state
For us everything is dull
Isnt it
Everytime i want to forget n enjoy
I am like you
I know darkness is just on the edge
Everytime
I want to enjoy
I hear those word
Those despicable words
Those unfair words
Those painful words
Everytime
I hear them echoo
Within me
" i am sorry sir u have blood cancer .....blood cancer.....blood cancerrr"
Hummphh
Sunset
Nothings beautiful about it
Nothing
..
.
..

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 थप पोस्ट

The DNA revolution: Crystallography and four-stranded DNA

[Dr. Sarah P. Gurung is a recent PhD graduate from University of Reading who lives in Reading (UK). Sarah originally comes from Balaju, Kathmandu, Nepal and moved to the UK when she was 15 years old.  Her PhD research includes the DNA revolution which is vital towards modern medical treatments. She shares her experience and the main idea of the study with Londonkathmandu.com]

The study and use of DNA has been even more interesting and vital for human treatments and other purposes today. The impossible of yesterday has become possible today. In 1953, Watson and Crick introduced us to their Nobel Prize winning model of DNA. We were shown how the molecule of life comes together as two intertwining strands to form a twisted-ladder structure. This discovery, based on the results obtained by Rosalind Franklin and her PhD student Raymond Gosling, opened doors to numerous fields in science, ranging from medicine to nanotechnology. 

The method that the student-supervisor pair used to study DNA is called X-ray Diffraction which is also the technique I used for the majority of my PhD. X-rays, are a form of electromagnetic radiation whose wavelengths are shorter than that of visible light. Therefore it can be used in many day to day applications that are limited to the naked eye, such as in airport security checks and medical CT scans; to see beyond what we cannot see. The German scientist Wilhelm Röntgen is credited for the discovery of X-rays in 1895. He called this then unknown radiation “X-radiation” which has been shortened to X-ray over the years.

Diffraction, on the other hand, is the bending of waves due to encountering an obstacle. It is just like how music coming from a radio in one room will sound different when heard from another room because the sound waves have to go through walls or the water waves will change in direction when you throw a pebble into a pond. Similarly, when X-rays hit an object, which was DNA in the case of Franklin and Gosling, the rays are diffracted or bend to different directions and positions. These positions can then be used as a map to determine the location of each atom within a molecule. This technique can be used to study molecules of a range of sizes, from small to larger biological ones.

The experiments and mathematical calculations conducted by Max von Laue, Paul Peter Ewald and the father-son duo William and Laurence Bragg between1912-1913 gave rise to X-ray Crystallography. They introduced the concept of using crystals as obstacles for X-ray diffraction. Imagine if I fall down a flight of stairs and I need to check if my arm is broken. I would go to a hospital and have an X-ray scan. The report would show me the condition of my solid bones but not that of my blood or other fluids. Similarly, hitting a DNA crystal with X-rays will show how the atoms come together inside. We can also test on DNA in its liquid state using X-rays, but the results will appear more complicated because liquid is free-flowing and it will give us various atomic positions whereas a solid crystal sample will give us more specific positions of the atoms.  

For the majority of my project, I worked on how to crystallise or solidify four-stranded DNA for X-ray diffraction studies. A double helix DNA involves pairing of two DNA bases, adenine (A) with thymine (T) and guanine (G) with cytosine (C). This rule is almost natural and prevalent in all living things. However, recent studies have shown that DNA sequences which are rich in G and C can form four-stranded DNA instead of the regular two-stranded structure (Figure 1). Furthermore, these sequences are found in “telomeres” which control the aging process and the promoter regions of cancer genes, suggesting that four-stranded DNA may contribute to regulating cancer. In the past five years as a PhD student and a postdoctoral research assistant, I studied on the factors that affect the stability of these four-stranded DNA. My work heavily focused on C-rich DNA or intercalated “i-motifs” which form more readily in acidic environments. This was initially a topic of great contradiction because the human body has a pH of 7.4 so it was argued that the ‘i-motif’ cannot be present in humans.

However, I managed to crystallise an ‘i-motif’ in pH 7.0, going against the theory that they only form in acidic conditions. Moreover, only just recently, Daniel Christ and his research group in Sydney, Australia, showed the formation of i-motif DNA structures in the nuclei of human cells. Therefore, interest in i-motifs and other four-stranded DNA structures are increasing due to their unique pH-specific properties and their potential regulatory role in the human genome.

In short, my ever-challenging PhD research centred around the idea of solidifying cancer DNA from the human body so that a more precise outcome can be obtained on the DNA’s structure. The results can then be used in the future to check what kinds of drugs/medicines can be used or developed to treat cancer.

 Sarah

Figure 1: (Left) The two-stranded “Double Helix” DNA which is prevalent in most living organisms. The DNA bases A (green) always pairs with T (red) and C (yellow) always pairs with G (blue). (Right) The four-stranded “i-motif” DNA which has the potential to form in cancer genes. It is made up of C DNA bases.

 

 

Tireed

Tireed is what i feel right now
"Its so graceful:"
"Its so beautiful:"
"Its so amazing:"
People would say
At this sight.
This sight that i am beholding.
This beautiful sight.
They are right on their terms.
It is beautiful.
This sunset.
This golden radiance it spread.
Everything became beautiful.
The leaves were gold.
The path was gold.
The clouds looked like fire.
The birds were happy
Birds:
Returning home
The animals were happy
From the day's food
And for the night's rest
All were enjoying
Only i couldnt.
Walking down the road alone
I hear laughs
I hear talks
Of them people
Happy people
Enjoying the sunset
Only i couldn't bring myself to be happy
"Loook mommy preetty"
A little boy said pointing at the setting sun
"Ohhh those eyes"
Those innocent ignorant eyes of that kid
All they knew was happiness
Its true that the sunset was beautiful
Only i couldnt think it that way
And so clouldnt you
Right:
My fren "sunset":
Though everbody is haappy
U and i know it.
Only u and i kno
Whats its like to know when ones brilliance is at end
Whats it like to be added set at the back
"Hummphh"
"Sunset"
There is nothing beautiful about it
Nothing at all
It just reminded that night is near by
Darkness is nearby
Its coming
Isnt it my fren.
You who can
Comprehend my state
For us everything is dull
Isnt it
Everytime i want to forget n enjoy
I am like you
I know darkness is just on the edge
Everytime
I want to enjoy
I hear those word
Those despicable words
Those unfair words
Those painful words
Everytime
I hear them echoo
Within me
" i am sorry sir u have blood cancer .....blood cancer.....blood cancerrr"
Hummphh
Sunset
Nothings beautiful about it
Nothing
..
.
..

MP Appreciates Nepalese Students in Reading

Matt Rodda, MP of Reading East, has appreciated the achievements of Nepalese students in their GCSE this year.  MP Rodda was speaking as chief guest at a programme organised by Nepalese Highly Skilled Migrant Forum Reading to honour the Nepalese community students completing their GCSE this year at the Warehouse, Cumberland Road in Reading on Sunday.

Nepalese students performed well in their GCSE this year from different schools of   Reading including Bulmershe, Reading Girls, Forest, Prospect and others.  A sample of Bulmershe school in Reading showed Nepalese students were nearly 10 % ahead of the average national level GCSE grade this year with 71.3 % achieving A*- C in the GCSE.   This was a remarkable progress of the students, who have a very short history of migration to the UK and are very new to the UK education system.

The sample data of the students' achievement shows, the percentage of achieving A*- has been increasing, while the percentage of lower level D-G grade has decreased drastically in the last few years.  A large number of students of the community have enrolled in renowned Universities for higher education in different subjects including the skills shortage areas like medicine, engineering, IT, science, teaching among others is also the evidence of the improvement.  

MP Rodda said he was very pleased with the remarkable progress made by the Nepalese community students even in a short period of time in the UK education system, and congratulated the students, parents and the whole community.

hsmpPhoto: Anupama Acharya

After being elected to the parliament in May this year, he said his focus is mainly on three issues facing our constituency and the country:  affordable housing, stop the cuts of Conservative government and fight for more funding to schools and national health services (NHS).  He said Labour government gave priority to the education so increased the funding to the schools during the labour government from 1997 to 2010, while the Conservatives have cut the school budget and there are fewer teachers and less funding to the school.

On behalf of parents, Mr Narayan Somai and Mrs Saraswati Khanal shared their experiences on how to help children to do well in their studies in an effective and responsible manner. We should create an environment of education so they learn themselves without pressurising them, they said.

Student Ayush Shrestha, shedding light on the new curriculum and new marking criteria which made difficult for students to get a higher grade this year, unveiled his GCSE exam preparation tips to achieve better results.

Coordinator of the GCSE Appreciation programme of the HSMP Forum  Chiranjibi Paudyal, presenting sample data of Nepalese students’ performance of Bulmershe school, said that the GCSE results was improving each and every year because of a number of factors including improvement in quality teaching and giving priority to education by Nepalese parents.

MP Rodda presented token of love to the GCSE students,  HSMP Forum Coordinator Dr Nabin Acharya gave away certificates while Sadiksha Giri, who was one of the top three students of Bulmershe school with 10 A* and one A in GCSE,  distributed Nepali flags to the students.

hsmp

More than 30 students completed the GCSE from different school this year.  Students, parents and community leaders were present at the programme held under the chairmanship of Bulmershe school student Sadiksha Giri, who was honoured for her excellent achievement in GCSE and inspire the younger generation.

HSMP Forum member Ramesh Kayasth conducted the programme, while co-ordinator of HSMP Forum Reading Dr Acharya extended vote of thanks to the guests and the participants before the conclusion of the programme. The Forum has been organising such programme for the last 7 years with a view to appreciating and celebrating the achievement of students and encouraging them for higher studies and making contributions to community and the country.

hsmp

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hsmp

Photo:Anupama Acharya

Beauty and lifestyle: A creation of my Study

Reading, UK
8 July  2017

I do not see myself as just a makeup artist, but an artist in general. I am currently studying graphics design at university and I started my youtube channel as part of a project for my course. The project was about the beauty of colours featured on different skin tones which aren't always shown in the beauty industry. 

The course I do lets me express my views in artistic ways & this freedom gives me the opportunity to raise awareness in what I find is important for my generation. This is why majority of my projects are shown through makeup and photography. I had a real interest in makeup before I studied Graphics Design which led me to combine the two so I could enjoy my course to its full potential. Instead of drawing on paper or using digital programs, I am basically using my face as a canvas and I love it. 

Having a youtube channel has improved my confidence which is shown through every video I post. As my confidence has grown, it has enabled me to share my work further and teach those who want to learn the art of makeup for themselves. The makeup looks I've created so far are what my supporters have asked to see.

I'm hoping that I can take my art to higher levels and one day, be able to collaborate with artists who have inspired me and my work. I also would love for the chance to put my country on the map & be known as a Nepalese artist. I have upcoming projects with different artists as the learning cycle never ends. I take great pride in my work and I'm very excited for what the future holds for me.

Youtube links: 

Please click here to view my Youtube Channel

My Pride My Motherland

At the twinkle of the Mt Everest
Shining ever, in peace and prosperity quest,
Proud to own You, my motherland Nepal
The land of treasure, once ruled by the Gopal

Lately freed of monarchy at people’s cost
Wept,but patient, at thousands lost
Generations sovereign, invincible my motherland
With gleaming swords at Bir Balbhadra’s hand

Swayambhu and Pashupati stand in symphony
Himal, Padad, Terai all embrace in harmony,
Your brave lovers today living global
 
Yet vibrantly with You, fostering overall 

My pride my motherland of all land
Whether New Zealand or Switzerland,
Flora and fauna all gather to one block
A top reason the loving tourists to flock

Rich in heritage, rich in hearts
Rich in shared humanly arts, 
Glad to have more Anuradha and Mahabir Pun
For the sake of glowing my adorable dune

Proud of ‘a whole’ Taplejung to Mahendranagar
As sober treasure again, Baglung or Biratnagar, 
Honesty and bravery symbol of the livelihood
All casts and creeds tales of the brotherhood

Always so proud, but upset I felt never
Yes, my motherland, in heart with me forever!

 

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